18752.The linking of antibiotic resistance gene with the plasmid vector became possible with:
Endonucleases
DNA ligase
Exonucleases
DNA polymerase
18753.In plant biotechnology, PEG is used in
Protoplast fusion
Cell culture preparation
Protoplast isolation
Hardening
18754.Polyethylene glycol method is used for
Energy production from sewage
Gene transfer without a vector
Biodiesel production
Seedless fruit production
18755.Genetic engineering has been successfully used for producing
Transgenic models for studying new treatments for certain cardiac diseases
Transgenic mice for testing safety of polio vaccine before use in humans
Animals like bulls for farm work as they have super power
Transgenic Cow-Rosie which produces high fat milk for making ghee
18756.Which of the following enzymes are used to join bits of DNA?
DNA polymerase
Primase
Ligase
Endonuclease
18757.Which one of the following bacteria has found extensive use in genetic engineering work in plants?
Bacillus coagulans
Xanthomonas citri
Clostridium septicum
Agrobacterium tumefaciens
18758.In double helix of DNA, the two DNA strands are
Coiled around a common axis
coiled around each other
coiled differently
Colied over protein sheath
18759.Restriction endonuclease
Restricts the synthesis of DNA inside the nucleus
Cuts the DNA molecule at specific sites
Cuts the DNA molecule randomly
Synthesizes DNA
18760.Transgenic plants are developed by
Introducing foreign genes
Introducing gene mutations
Deleting certain chromosomes parts
Stopping spindle formation
18761.A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of
Trichoderma
Pseudomonas
Bacillus
Xanthomonas
18762.The vector for T-DNA is
Thermus aquaticus
Salmonella typhimurium
Agrobacterium tumefaciens
Bacillus thuringiensis
18763.Cellular totipotency is demonstrated by
All plant cells
Only gymnosperm cells
All eukaryotic cells
Only bacterial cells
18764.Crop plants grown in monoculture are
Characterized by poor root system
Free from intraspecific competition
Low in yield
Highly prone to pests
18765.Differentiation of organs and tissues in a developing organism, is associated with
Differential expression of genes
Developmental mutations
Deletion of genes
Lethal mutations
18766.Micropropagation is a technique
for production of true to type plants
for production of haploid plant
for production of Somatic hybrids
for production of Somaclonal plants
18767.What is true of plasmid?
Part of nuclear chromosome
Contains genes for vital activities
Found in viruses
Widely used in gene transfer
18768.Somaclonal variation appears in plants
Transformed by recombinant DNA technology
Growing in polluted soil or water
Exposed to gamma rays
Raised in tissue culture
18769.Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells)
An antifeedant
A particular hormone
Both sense and anti-sense RNA
A toxic protein
18770.Plasmids are suitable vectors for gene cloning because
these can shuttle between prokaryotic and eukaryotic cells
these are small circular DNA molecules with their own replication origin site
these are small circular DNA molecules, which can integrate with host chromosomal DNA
these often carry antibiotic resistance genes
18771.Some of the characteristics of Bt cotton are
Medium yield, long fibre and resistance to beetle pests
Long fibre and resistance to aphids
High yield and resistance to bollworms
High yield and production of toxic protein crystals which kill dipteran pests
18772.Nucleosides are
Sugar + Phosphoric acid
Purine / Pyrimidine + Sugar + Phosphate
Purine / Pyrimidine + Phosphoric acid
Purine / Pyrimidine + Sugar
18773.Cultivation of Bt cotton has been much in the news. The prefix Bt means ______.
produced by biotechnology using restriction enzymes and ligases
bigger thread variety of cotton with better tensile strength
barium-treated cotton seeds
carrying an endotoxin gene from Bacillus thuringiensis
18774.Stirred-tank bioreactors have been designed for
Purification of the product
Addition of preservatives to the product
Availability of oxygen throughout the process
Ensuring anaerobic conditions in the culture vessel
18776.Which of the following terms is used to describe the component isolated from a plant, for in vitro culturing in the specific medium?
Synthetic seeds
Embryoid
Callus
Explant
18778.Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?
Exonuclease
Protease
Lipase
Endonuclease
18779.The enzyme employed for amplification of DNA during PCR is commercially obtained from
Trichoderma reesei
Bacillus licheniformis
Streptococcus pyogenes
Thermus aquaticus
18780.A regulatory body working under MoEF for the release of transgenic crops is
NBPGR
GEAC
NSC
NIPGR
18781.Cry1 endotoxins obtained from Bacillus Thuringiensis are effective against:
Flies
Mosquitoes
Boll worms
Nematodes
18782.In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors
Statements
(A) A bacterium
(B) Plasmid
(C) Plasmodium
(D) Bacteriophage
(A), (B) and (D) only
(A) only
(A) and (C) only
(B) and (D) only
18783.Read the following four statements (A-D) about certain mistakes in two of them
(A) The first transgenic buffalo Rosie produced milk which was human alpha-lactalbumin enriched
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules
(C) Downstream processing is one of the steps of R-DNA technology
(D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host
Which are the two statements having mistakes?
Statements (A) and (C)
Statements (A) and (B)
Statements (B) and (C)
Statements (C) and (D)
18784.Cry II Ab and Cry I Ab produce toxins that control
Tobacco budworms and nematodes respectively
Corn borer and cotton bollworms respectively
Cotton bollworms and corn borer respectively
Nematodes and tobacco budworms respectively
18785.Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein
Binds with epithelial cells of midgut of the insect pest ultimately killing it
Does not kill the carrier bacterium which is itself resistant to this toxin
Is activated by acid pH of the foregut of the insect pest
Is coded by several genes including the gene cry
18786.What is true about Bt toxin?
Bt protein exists as active toxin in the Bacillus
The inactive protoxin gets converted into active form in the insect gut
The concerned Bacillus has antitoxins
The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication
18787.
Assertion : In plant tissue culture, somatic embryos can be induced from any plant cell.
Reason : Any viable plant cell can differentiate in to somatic embryos.
Both Assertion and Reason are true and the Reason is the correct explanation of the Assertion.
Both Assertion and Reason are true but the Reason is not the correct explanation of the Assertion.
Assertion is true statement but Reason is false.
Both Assertion and Reason are false statements.
18788.Which one of the following is a correct statement?
The anticoagulant hirudin is being produced from transgenic Brassica napus seeds
Somatic hybridization involves fusion of two complete plant cells carrying desired genes
"Bt" in "Bt-cotton" indicates that it is a genetically modified organism produced through biotechnology
"Flavr Savr" variety of tomato has enhanced the production of ethylene which improves its taste
18789.Bacillus thuringiensis is used to control
Nematodes
Fungal pathogens
Bacterial pathogens
Insect pests
18790.The genetically-modified (GM) brinjal in India has been developed for
Enhancing shelf life
Insect-resistance
Drought-resistance
Enhancing mineral content
18792.The protein products of the following Bt toxin genes cry IA c and cry II Ab are responsible for controlling
Bollworm
Roundworm
Moth
Fruit fly
18793.Variable number of tandem repeats (VNTRs) in the DNA molecule are highly useful in
Monoclonal antibody production
DNA fingerprinting
Recombinant DNA technology
Stemcell culture
18794.Silencing of mRNA has been used in producing transgenic plants resistant to
Bollworms
Bacterial blights
White rusts
Nematodes
18795.Restriction endonucleases are most widely used in recombinant DNA technology. They are obtained from
Bacteriophages
Bacterial cells
Plasmids
All prokaryotic cells
18796.PCR proceeds in three distinct steps governed by temperature, they are in order of
Annealing, Synthesis, Denaturation
Synthesis, Annealing, Denaturation
Denaturation, Annealing, Synthesis
Denaturation, Synthesis, Annealing
18797.Which of the following is correctly matched?
Ligase - Molecular scissors
Thermus aquaticus - Bt-gene
Agrobacterium tumefaciens - Tumour
Hind II - Plasmid vector
18798.Sterilization of tissue culture medium is done by
mixing the medium with antifungal agents
filtering the medium through fine sieve
autoclaving of medium at 120° for 15 min
keeping the medium at –20°C
18799.The process of RNA interference has been used in the development of plants resistant to
Insects
Nematodes
Fungi
Viruses
18800.An improved variety of transgenic basmati rice
Gives high yield and is rich in vitamin A
Does not require chemical fertilizers and growth hormones
Give high yield but has no characteristic aroma
Is completely resistant to all insect pests and diseases of paddy
18801.Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
5′ CACGTA 3′; 3′ CTCAGT 5′
5′ CGTTCG 3′; 3′ ATGGTA 5′
5′ GATATG 3′; 3′ CTACTA 5′
5′ GAATTC 3′; 3′ CTTAAG 5′